Table 1.
Primer sequences and probes used in real-time PCR assay
| Gene | Primer and probe sequence (5′to3′) | Product length(bp) | GenBank |
|---|---|---|---|
| EV71 |
(R) CCCGCTCTGCTGAAGAAACT |
89 |
AF302996 |
| |
(F)AGTGATGAGAGTATGATTGAGACACG |
|
|
| |
(P) TCGCACAGCACAGCTGAGACCACTC |
|
|
| β-actin |
(R) CAAGTACTCCGTGTGGATCG |
90 |
NM_001101.3 |
| |
(F) GGATGCAGAAGGAGATCACTG |
|
|
| |
(P) CCCTGGCACCCAGCACAATGA |
|
|
| RIG-1 |
(R) CCTCTGCACTGTTGCTCAGGAC |
192 |
NM_004585.3 |
| |
(F) CTCTTGGCTTCGAGATGGCTTC |
|
|
| MDA5 |
(R) ATTGGTGACGAGACCATAACGGATA |
196 |
NM_022168.2 |
| |
(F) AGGAGTCAAAGCCCACCATCTG |
|
|
| TLR2 |
(R) CACAAAGTATGTGGCATTGTCCAG |
158 |
NM_003264.3 |
| |
(F) GTGTTGCAAGCAGGATCCAAAG |
|
|
| TLR3 |
(R) AGTGCCGTCTATTTGCCACACA |
181 |
NM_003265.2 |
| |
(F) AACAGTGCACTTGGTGGTGGAG |
|
|
| TLR4 |
(R) ATGCGGACACACACACTTTCAAATA |
143 |
NM_138554.3 |
| |
(F) TTGAGCAGGTCTAGGGTGATTGAAC |
|
|
| TLR6 |
(R) AGCCTTCAGCTTGTGGTACTTGTTG |
138 |
NM_006068.2 |
| |
(F) CAGAGTGAGTGGTGCCATTACGA |
|
|
| TLR7 |
(R) TCTTCAACCAGACCTCTACATTCCA |
172 |
NM_016562.3 |
| |
(F) GGAACATCCAGAGTGACATCACAG |
|
|
| TLR8 |
(R) GATTGCTGCACTCTGCAATAACTGA |
196 |
NM_138636.3 |
| |
(F) GCTGCTGCAAGTTACGGAATGA |
|
|
| TLR9 |
(R) CAGGGCCTTCAGCTGGTTTC |
97 |
NM_017442.2 |
| |
(F) CCGTGACAATTACCTGGCCTTC |
|
|
| TLR10 |
(R) TGTGTTGCAAGATAATTCGTGGAGA |
103 |
NM_001017388.1 |
| |
(F) CATGATGGTTGGATGGTCAGATTC |
|
|
| β-actin |
(R) CTAAGTCATAGTCCGCCTAGAAGCA |
186 |
NM_001101.3 |
| (F) TGGCACCCAGCACAATGAA |