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. 2012 Dec 10;7(12):e51786. doi: 10.1371/journal.pone.0051786

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A) Cell proliferation assay to compare the growth over time (days, d) of the parental MCF10A-1 and vector control cells with single-cell-derived SATB1-overexpressing MCF10A-1 clones clone-1 and clone-2 on plastic dishes (2D) or on Matrigel (3D). Error bars indicate ±s.e.m. from three independent experiments. B) Representative photographs of soft agar colonies formed by control and SATB1 overexpressing (clone-1 and clone-2) MCF10A-1 cells after 25 days of culture. The mean colony counts from three replicates are shown. C) Immunoblot analyses for the expression of mesenchymal markers (fibronectin and vimentin), epithelial markers (E-cadherin and ß-catenin) as well as SATB1 target ERBB2 in MDA-MB-231 (parental, control and SATB1-depleted by shRNA1 or shRNA2) and MCF10A-1 (parental, control, clone-1 and clone-2). Cell lysates were prepared from cells cultured on plastic dishes (2D). GAPDH was used as a loading control.