Figure 3. Native and unfoldon ELISA assays for viruses and vaccines using polyclonal antisera and monoclonal antibodies.

The viruses and vaccines were diluted to equivalent hemagglutination units (HAU) although not comparable for detergent-released HA in vaccines versus virus particles and the H7 vaccine had no HAU. Analysis of the binding curves gives total binding sites (Bmax) (Figure 3A) and overall avidity (Kd) (Figure 3B).