Table 2. Dendritic Cell Maturation and Cytokine Secretion by T Cells Stimulated in vitro with Influenza A Virus Vaccines and Hemagglutinin Proteins.1 .
| Maturation3 | IFNγ4 | IL-44 | |||
| Stimulator2 | Initial Test | After Dialysis5 | Initial Test | After Dialysis5 | Initial Test |
| Vaccine | |||||
| H5N1 | |||||
| A/VN/04 (SP) | NT6 | Yes | NT6 | >105 | NT6 |
| A/Indo/05 (SP) | No | Yes | 0 | >105 | 0 |
| H7N7 | |||||
| A/Mallard/00 (SP) | Yes | >105 | 104 | ||
| H9N2 | |||||
| A/HK/G1/99 (Wyeth) | Yes | >105 | >104 | ||
| A/CK/G9/97 (Novartis) | Yes | >105 | >104 | ||
| H1N1 | |||||
| A/Cal/09 (CSL) | No | Yes | 0 | >105 | 0 |
| rHA | |||||
| H5N1 | |||||
| A/VN/04 | Yes | >104 | >104 | ||
| A/Indo/05 | Yes | >104 | >104 | ||
| H7N7 | |||||
| H7N7 A/Mallard/00 | Yes | >104 | >104 | ||
| H9N2 | |||||
| A/HK/G1 | No | Yes (low) | 0 | >104 | 0 |
| A/CK/G9 | Yes | Yes | >104 | >104 | >104 |
| H1N1 | |||||
| A/Cal/09 | Yes | >105 | >104 | ||
1
Immature dendritic cells loaded with antigen, matured with cytokines and used to present antigens to T cells that secrete cytokines.
2
Monovalent vaccine (manufacturer) or recombinant HA proteins described in M&M.
3
As determined in FACS for CD83 and HLADR.
4
Numbers of ELISpots for indicated cytokine secretion after T cell stimulation; unstimulated lymphocytes, DC only and medium controls did not show ELISpots.
5
The test was before and after dialysis in a repeat test, after dialysis result shown.
6
NT = not tested.