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. 2012 Oct 2;10:82. doi: 10.1186/1741-7007-10-82

Table 2.

Confidence score of homologs from Swiss-Prot database.

Evaluation method Criteria Points
BLAST e-value < 0.001 1

Sequence identity between the query and the hit identity 30% to 50%, coverage > 40% 1
identity 50% to 70%, coverage > 40% 2

identity 70% to 90%, coverage > 40% 3

identity 90% to 99%, coverage > 40% 4

identity > 99%, coverage > 40% 5

BLAST alignment coverage for both query and hit 60% to 80% 1

80% to 100% 2

The query against the proteome associated with the hit Best hit 2

N/A 1

The hit against the proteome associated with the query Best hit 2

N/A 1