Table 3.
Confidence score of predicted gene ontology terms
| Evaluation method | Criteria | Points |
|---|---|---|
| BLAST e-value | 0.001 | 1 |
| Sequence identity between the query and the hit | identity 30% to 50%, coverage > 40% | 1 |
| identity 50% to 70%, coverage > 40% | 2 | |
| identity 70% to 90%, coverage > 40% | 3 | |
| identity 90% to 100%, coverage > 40% | 4 | |
| Alignment coverage for query and hit | 60% to 80% | 1 |
| 80% to 100% | 2 | |
| Evidence code of the GO term assigned to the hit | EXP, IDA | 3 |
| IPI, IMP, IGI, IEP, ISO, TAS | 2 | |
| ISS, ISA, ISM, IGC, IBA, IBD, IKR, IRD, RCA, NAS, IC, IEA | 1 | |
| Consensus bonus | Associated with no less than three hits | 2 |
EXP: inferred from experiment; GO: Gene Ontology; IBA: inferred from biological aspect of ancestor; IBD: inferred from biological aspect of descendant; IC: inferred by curator; IDA: inferred from direct assay; IEA: inferred from electronic annotation; IEP: inferred from expression pattern; IGC: inferred from genomic context; IGI: inferred from genetic interaction; IKR: inferred from key residues; IMP: inferred from mutant phenotype; IPI: inferred from physical interaction; IRD: inferred from rapid divergence; ISA: inferred from sequence alignment; ISM: inferred from sequence model; ISO: inferred from sequence orthology; ISS: inferred from sequence or structural similarity; NAS: non-traceable author statement; RCA: inferred from reviewed computational analysis; TAS: traceable author statement.