Table 2. Oligonucleotide primers and amplified PCR products in the study.
Primer | Dye | Oligonucleotide sequences (5′-3′) | Target | Amplicon (bp) | Reference |
2851-80 | PET | GTGCTTGGGTCTTTTCTCTG | Phage-sbcB left junction | 914 | [26] |
sbcB-R2 | None | TCCAGGCGTAAGGATCGTAG | This study | ||
sbcB-F | VIC | GACAGCAGAAACAACGGATTTAAC | Phage-sbcB right junction | 730 | This study |
2851-82 | None | CCAGCGTGGGATAAAAGAGAATC | [26] | ||
sbcB-F | VIC | GACAGCAGAAACAACGGATTTAAC | sbcB insertion site | 406 | This study |
sbcB-R2 | None | TCCAGGCGTAAGGATCGTAG | This study | ||
F | 6FAM | CACCGGAAGGACAATTCATC | Phage-yehV left junction | 824 | [10] |
B | None | AACAGATGTGTGGTGAGTGTCTG | [10] | ||
A | PET | AAGTGGCGTTGCTTTGTGAT | Phage-yehV right junction | 702 | [10] |
E | None | GATGCACAATAGGCACTACGC | [10] | ||
A | PET | AAGTGGCGTTGCTTTGTGAT | Phage-yehV variant right junction | 295 | [10] |
E3 | None | AGCGATACAGATCTCAACAC | This study | ||
A | PET | AAGTGGCGTTGCTTTGTGAT | yehV insertion site | 340 | [10] |
B | None | AACAGATGTGTGGTGAGTGTCTG | [10] | ||
C | 6FAM | AGGAAGGTACGCATTTGACC | Phage-wrbA right junction | 592 | [10] |
G | None | ATCGTTCGCAAGAATCACAA | [10] | ||
H | PET | CCGACCTTTGTACGGATGTAA | Phage-wrbA left junction | 506/537 | [10] |
D | None | CGAATCGCTACGGAATAGAGA | [10] | ||
C | 6FAM | AGGAAGGTACGCATTTGACC | wrbA insertion site | 314 | [10] |
D | None | CGAATCGCTACGGAATAGAGA | [10] | ||
argW-A | VIC | CCGTAACGACATGAGCAACAAG | Phage-argW right junction | 583 | This study |
argW-B | None | GCAGTATCACGCAGAGCTGAAG | This study | ||
argW-C | 6FAM | GCATCTCACCGACGATAACA | Phage-argW left junction | 462 | This study |
argW-D | None | AATTAGCCCTTAGGAGGGGC | This study | ||
argW-A | VIC | CCGTAACGACATGAGCAACAAG | argW insertion site | 216 | This study |
argW-D | None | AATTAGCCCTTAGGAGGGGC | This study | ||
Stx1-2 | VIC | CACAGACTGCTGCAGTGAGG | Stx1 | 475 | [20] |
Stx1-1 | None | CAGTTAATGTGGTGGCGAAG | [20] | ||
stx2-1072F | NED | AGGATGACACATTTACAGTGAAGGTT | Stx2a -specific | 126 | [50] |
stx2-1197R | None | CACAGGTACTGGATTTGATTGTGAC | [50] | ||
stx2c-F858 | NED | CGACAGGCCCGTTATAAAAA | Stx2c -specific | 243 | This study |
stx2c-R1100 | None | GGCCACTTTTACTGTGAATGTATC | [5] |