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. 2012 Dec 11;7(12):e51572. doi: 10.1371/journal.pone.0051572

Table 2. Oligonucleotide primers and amplified PCR products in the study.

Primer Dye Oligonucleotide sequences (5′-3′) Target Amplicon (bp) Reference
2851-80 PET GTGCTTGGGTCTTTTCTCTG Phage-sbcB left junction 914 [26]
sbcB-R2 None TCCAGGCGTAAGGATCGTAG This study
sbcB-F VIC GACAGCAGAAACAACGGATTTAAC Phage-sbcB right junction 730 This study
2851-82 None CCAGCGTGGGATAAAAGAGAATC [26]
sbcB-F VIC GACAGCAGAAACAACGGATTTAAC sbcB insertion site 406 This study
sbcB-R2 None TCCAGGCGTAAGGATCGTAG This study
F 6FAM CACCGGAAGGACAATTCATC Phage-yehV left junction 824 [10]
B None AACAGATGTGTGGTGAGTGTCTG [10]
A PET AAGTGGCGTTGCTTTGTGAT Phage-yehV right junction 702 [10]
E None GATGCACAATAGGCACTACGC [10]
A PET AAGTGGCGTTGCTTTGTGAT Phage-yehV variant right junction 295 [10]
E3 None AGCGATACAGATCTCAACAC This study
A PET AAGTGGCGTTGCTTTGTGAT yehV insertion site 340 [10]
B None AACAGATGTGTGGTGAGTGTCTG [10]
C 6FAM AGGAAGGTACGCATTTGACC Phage-wrbA right junction 592 [10]
G None ATCGTTCGCAAGAATCACAA [10]
H PET CCGACCTTTGTACGGATGTAA Phage-wrbA left junction 506/537 [10]
D None CGAATCGCTACGGAATAGAGA [10]
C 6FAM AGGAAGGTACGCATTTGACC wrbA insertion site 314 [10]
D None CGAATCGCTACGGAATAGAGA [10]
argW-A VIC CCGTAACGACATGAGCAACAAG Phage-argW right junction 583 This study
argW-B None GCAGTATCACGCAGAGCTGAAG This study
argW-C 6FAM GCATCTCACCGACGATAACA Phage-argW left junction 462 This study
argW-D None AATTAGCCCTTAGGAGGGGC This study
argW-A VIC CCGTAACGACATGAGCAACAAG argW insertion site 216 This study
argW-D None AATTAGCCCTTAGGAGGGGC This study
Stx1-2 VIC CACAGACTGCTGCAGTGAGG Stx1 475 [20]
Stx1-1 None CAGTTAATGTGGTGGCGAAG [20]
stx2-1072F NED AGGATGACACATTTACAGTGAAGGTT Stx2a -specific 126 [50]
stx2-1197R None CACAGGTACTGGATTTGATTGTGAC [50]
stx2c-F858 NED CGACAGGCCCGTTATAAAAA Stx2c -specific 243 This study
stx2c-R1100 None GGCCACTTTTACTGTGAATGTATC [5]