Figure 5. Expression analyses and histone H3 methylation pattern changes of CnABI3 in yellow-cedar.

(A) Embryos (B) Megagametophytes. nChIP/qPCR (left column) and expression analyses (right column). Averages of three biological replicates are shown +/− SE. Mature yellow-cedar seeds were subjected to a full (12-week) dormancy-breaking treatment, or the full dormancy-breaking treatment followed by 1 day in germination conditions (“germinating”). Seedlings were at an early stage (4 mm radicle length, and greened cotyledons). A control treatment consisted of maintaining seeds in warm, moist conditions for 12 weeks, which did not break dormancy.