Table 1. Properties of mMaple and related variants.
Protein name | State | λex(nm) | λem(nm) | εa | Φ | Brightnessb | pK a |
mMaple | green | 489 | 505 | 15 (26, 59) | 0.74 | 11 | 8.2 |
mMaple | red | 566 | 583 | 30 | 0.56 | 17 | 7.3 |
mClavGR2 | green | 488 | 504 | 19 (25, 60) | 0.77 | 15 | 8.0 |
mClavGR2 | red | 566 | 583 | 32 | 0.54 | 17 | 7.3 |
mEos2 | green | 506 | 519 | 78 (32, 95) | 0.43 | 34 | 5.6c |
mEos2 | red | 573 | 584 | 39 | 0.35 | 14 | 6.4c |
Extinction coefficent (mM−1 cm−1) at peak absorbance wavelength in PBS (pH 7.4). Value in parentheses was determined at pH 4 and pH 10, respectively.
Product of ε and Φ in mM−1 cm−1. For comparison, the brightness of EGFP and mCherry are 34 mM−1 cm−1 and 16 mM−1 cm−1, respectively [53].
Data from McKinney et al. [30].