Figure 5. All three clonal lineages of Toxoplasma inhibit STAT1-mediated gene expression. A, B.

A HEK293 GAS luciferase reporter cell line was infected with RH(I), RHΔrop16, GT1(I), Pru(II), PruΔgra15, ME49(II), CEP(III), or VEG(III) parasites, or left uninfected, and subsequently stimulated, or not, with 100 U/ml IFNγ. Cells were then lysed and luciferase activity was measured. Results are from 2–8 experiments per condition, with a pre-infection time of 1–5 hours followed by a stimulation of 12–24 hours, and represent the average induction over uninfected, unstimulated samples. Error bars represent SEM. Asterisk (*) indicates p<0.05 compared to uninfected cells in the same condition.