Figure 11. Location and identity of BrdU-labeled cells in cochlear cultures.
Frozen sections of cultured cochleae typically contained BrdU-labeled cells (green) in the sensory epithelium and within the basilar membrane (arrows, A, B). Cell nuclei are labeled with DAPI (blue). (C, D) Confocal images of cochleae that were double labeled for KUL01 (green, macrophages) and BrdU (red) indicate that macrophages constituted a minority of proliferating cells (arrows). Numerous quiescent macrophages (arrow heads) were also present. Scale bar (for images C and D) = 50 µm.