Figure 3. Endodermal pouch morphogenesis is affected in vgll2a morphants.
Single confocal, Z-plane images of an uninjected (A) and vgll2a morphant embryo (B), stained with the zn-8 antibody, marking the endodermal pouches in lateral views. Uninjected embryos (A) exhibit elongated endodermal pouches, numbered 1 – 5, formed by tight folds of epithelium at 38 hpf. In contrast, the endodermal pouches of embryos injected with 5 ng vgll2a ATG MO (B) are shorter and wider, and the epithelial cells appear less organized relative to uninjected controls. Anterior is to the left in both panels.