Figure 5. A TLR7-Dependent Mechanism Detects Retroviruses.
(A) WT I/LnJ and Myd88−/− I/LnJ mice were i.p. injected with MMTV. Sera were analyzed for reactivity against MMTV virion proteins by immunoblot 10 weeks after infection.
(B) Sera from RL-MuLV-infected B6 (left) and I/LnJ (right) mice with indicated genotypes were tested for reactivity against MuLV virion proteins by immunoblot 10 weeks after infection. PFU × 102: virus titers in mouse spleens (105 cells) 10 weeks after infection.
(C) WT I/LnJ and Tlr7−/− I/LnJ mice were i.p. injected with MMTV. Sera were analyzed for reactivity against MMTV virion proteins by immunoblot 10 weeks after infection.
Goat anti-mouse Abs coupled to HRP were used at the second step. Numbers correspond to individual mice within a single experiment.