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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Chromosoma. 2012 Oct 14;121(6):629–642. doi: 10.1007/s00412-012-0387-4

Figure 6.

Figure 6

Calpain inhibition induces coilin foci and alters cleaved coilin fragment levels. (A and B) ALLN treatment induces coilin foci in U2OS and WI-38 cells. U2OS and WI-38 cells were treated with ALLN or DMSO (vehicle) for 16 hr. The treated cells were fixed and immunostained for coilin (green), SMN (red) and nuclei (DAPI) (blue). Coilin, SMN and DAPI images were overlaid in merge panel. In U2OS and WI-38 cells, arrows indicate cells with predominantly nucleoplasmic coilin, arrowheads indicate coilin foci with SMN (Cajal body) and double arrowheads mark coilin foci without SMN (A). More than 100 cells were counted for each treatment and cell type and scored for the presence or absence of coilin foci. These percentages are presented in (B). (C) ALLN treatment decreases cleaved coilin fragment levels in WI-38 cells. U2OS and WI-38 cells were treated with DMSO or ALLN for 16 hr. Also shown is 16 hr cisplatin treatment of WI-38 cells. Cell lysates were subjected to SDS-PAGE, Western transfer, and blots probed with anti-coilin antibodies H300 (upper panel) and anti-β-tubulin antibodies (lower panel). Results are representative of three independent experiments.