Figure 7.

Calpain 4 knockdown abrogates cisplatin-induced nucleolar accumulation of coilin. U2OS cells were transfected with control or calpain-targeted siRNA. 32 hr post-transfection, cells were treated with cisplatin or left untreated. 48 hr post-transfection, cells were fixed and immunostained for coilin (green), calpain 4 (red) and nuclei were stained with DAPI (blue). The arrow indicates cells with normal CB coilin localization, arrowhead indicates cell with predominantly nucleolar coilin, double arrowhead indicates cells with predominantly nucleoplasmic coilin and double arrow indicates cells with small coilin foci.