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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Chromosoma. 2012 May 9;121(6):565–572. doi: 10.1007/s00412-012-0375-8

Figure 4. Comparing the early and late mitotic competencies of Plk1T210D via chemical genetics.

Figure 4

a PLK1Δ/Δ cells expressing Plk1wt or Plk1T210D (fused to mCherry) and/or GFP-tagged Plk1as were processed for Western blotting with the indicated antibodies. b Cells were grown for 6 days in the presence of 0, 0.078, 0.313, 1.25, 5, or 20 μM 3MB-PP1 before fixation and staining with crystal violet. c Cells were treated with nocodazole ± 10 μM 3MB-PP1 for 12 hours, then harvested for Western blotting. d Cells were treated with 3-MB-PP1 30 minutes after monastrol washout, then fixed and stained with antibodies to pS170-HsCYK-4 (green), RhoA (red), or DAPI (blue). Scale bar represents 10 microns.