Figure 2.

Angiogenic and cardiomyocyte responses to progressive left ventricular hypertrophy (LVH). (a,e–h) Progressive LVH triggered endogenous angiogenesis seen as enlarged capillaries. (b) Ten weeks after TAC operation, the number of capillaries per myocyte was significantly increased. Progressive LVH was seen as (c) decreased number of capillaries per mm² 2 weeks after the operation and (d) cardiomyocytes per mm² in all time points. Quantification was done from five endothelium-stained microscopic fields from each animal at ×400 magnification. Mean ± SEM, n = 6/group (in a–d), statistical analyses with one-way analysis of variance and Bonferroni's multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001. Representative pictures from endothelial stainings with Biotinylated Griffonia (Bandeiraea) Simplicifolia Lectin I, bars 50 µm (in e–h). TAC, transversal aortic constriction.