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. 2012 Sep 11;20(12):2335–2346. doi: 10.1038/mt.2012.182

Figure 4.

Figure 4

Human ES and induced pluripotent stem (iPS) cells differentiated with human fetal lung conditioned medium resulted in enhanced survivability and improved lung physiological functions in bleomycin lung injury model. (a) Kaplan–Meier curve showing survival rate of mice transplanted with phosphate-buffered saline (PBS), 293-CM differentiated or human fetal lung conditioned medium (HFL-CM) differentiated human ES cells over a 3 weeks period after bleomycin-induced lung injury. Transplantation study was performed five times with eight mice in each experimental group using human embryonic stem (hES) cells differentiated with HFL-CM, PBS, or 293-CM differentiated cells as controls. The P values between the experimental groups are as shown. (b) Kaplan–Meier curve showing survival rate of mice transplanted with hiPS cells differentiated with HFL-CM, PBS, or 293-CM differentiated cells into bleomycin-injured mice. The experiment was performed twice with five mice in each of the experimental groups. The survival analysis was performed using Kaplan–Meier estimator. The P values between the experimental groups are as shown. (c) Measurement of lung functions by whole-body plethysmography of mice transplanted with hES cells differentiated with HFL-CM, PBS, or 293-CM differentiated cells. Graphical representation showing breathing rates and tidal volumes of the three groups of mice measured over a 3-week period. Error bars represent SD obtained from remaining mice at each time point.