Table 2.

Compositions of liposomes labeled with ⁶⁴Cu (H–K) and ¹¹¹In (L–P). Liposomes H, I, L, M and N were formulated using DEG3SL 16 then labelled with ⁶⁴Cu or ¹¹¹In post-formulation. Liposomes J, K, O and P were formulated using DEG6SL 17 then labelled with ⁶⁴Cu or ¹¹¹In post-formulation. Liposomes Q and R were formulated as controls lacking the DOTA-lipids. Samples L, N, O and P were used for in vivo biodistribution.

Liposome	DOPE	Cu/In-lipid	PEG-lipid	DOTMA	FL-DHPE	DLS (PDI)	Zeta
H	10%	30% Cu-DEG3SL	50% DODEG4	10%	–	–	–
I	10%	30% Cu-DEG3SL	–	60%	–	–	–
J	10%	30% Cu-DEG6SL	50% DODEG4	10%	–	–	–
K	10%	30% Cu-DEG6SL	–	60%	–	–	–
L	9%	30% In-DEG3SL	50% DODEG4	10%	1%	73.9 nm (0.2)	(+) 54 mV
M	10%	30% In-DEG3SL	–	60%	–	–	–
N	2%	30% In-DEG3SL	7% DSPE-PEG2000	60%	1%	141.7 nm (0.2)	(+) 43 mV
O	9%	30% In-DEG6SL	50% DODEG4	10%	1%	70.1 nm (0.2)	(+) 50 mV
P	2%	30% In-DEG6SL	7% DSPE-PEG2000	60%	1%	142.6 nm (0.2)	(+) 31 mV
Control Q	40%	–	50% DODEG4	10%	–	–	–
Control R	50%	–	–	50%	–	–	–