Fig. 4.

Mutation of Crg2 but not Crg1 impacts the mass of the infected mouse lung tissue (A) and fungal burden throughout the disease process (B). Mice infected through the intranasal route were monitored for 2, 7 and 14 days following infection. Mice were sacrificed at indicated time points and CFU assay performed on brain, kidney and lung. No viable C. neoformans was recovered from the brain or kidneys of infected animals during this period of time. Large variations in mass of the lung suggest varying degrees of inflammation, and a steady decline in CFU over 2–14 days indicates the necessity of Crg2 for robust infection in the lung, n = 5.