Figure 2.

Treatment with rapamycin increases the absolute number and mean IFN-γ production (on a per cell basis) of splenic site IV-specific T_CD8, but not those of alloreactive T_CD8. Splenocytes from vehicle- and rapamycin-treated B6 mice that were immunized with KD2SV cells were restimulated ex vivo with peptides corresponding to T Ag epitopes (sites I, II/III, IV or V), syngeneic T Ag⁺ C57SV cells, or allogeneic KD2SV cells. The frequency of cognate IFN-γ⁺ T_CD8 was used to enumerate the absolute number of these cells within each spleen (A), and their MFI for IFN-γ was also determined using FlowJo software (B). Data are shown as mean ± SEM obtained from 19 mice/group pooled from 5 independent experiments.