Fig. 4.

Analyses of plasma of apoA-I^−/−, apoA-I^−/− × apoE^−/−, ABCA1^−/−, and LCAT^−/− mice infected with the adenovirus expressing the human apoA-IV by density gradient ultracentrifugation and SDS-PAGE, EM, and two-dimensional gel electrophoresis. A–D: SDS-PAGE analysis of density gradient ultracentrifugation fractions. E–H: EM pictures of HDL fractions 6–7 obtained from mice expressing human apoA-IV following density gradient ultracentrifugation of plasma, as indicated. The photomicrographs were taken at 75,000× magnification and enlarged three times. I–M: Analysis of plasma obtained from mice expressing the human apoA-IV following two-dimensional gel electrophoresis and Western blotting. A, E, I: Analyses of apoA-I^−/− mice. B, F, J: Analyses of apoA-I^−/− × apoE^−/− mice. C, G, K: Analyses of ABCA1^−/− mice. D, H, L, M: Analyses of LCAT^−/− mice.