TABLE 1.
Diet composition of treatment diets
| AIN-76A | AIN-76A Modified | 6%-SF Diet | 12%-SF Diet | 24%-SF Diet | |
| Ingredient (g/kg) | |||||
| Casein | 200 | 200 | 165 | 165 | 165 |
| DL methionine | 3 | 3 | 3 | 3 | 3 |
| Lard | 0 | 0 | 3.3 | 35.4 | 68.6 |
| Coconut oil | 0 | 0 | 1 | 30 | 96.7 |
| Corn oil | 50 | 15.6 | 62.5 | 49.9 | 25.2 |
| Soybean oil | 0 | 3.6 | 14.1 | 9.3 | 2.5 |
| Olive oil | 0 | 30.9 | 122.1 | 78.4 | 10 |
| Corn starch | 0 | 80 | 50 | 50 | 50 |
| Maltodextrin | 0 | 100 | 100 | 100 | 100 |
| Sucrose | 500 | 469.5 | 381.5 | 381.5 | 381.5 |
| Cellulose | 50 | 50 | 50 | 50 | 50 |
| Vitamin mix (AIN-76A) | 10 | 10 | 10 | 10 | 10 |
| Mineral mix (AIN-76A) | 35 | 35 | 35 | 35 | 35 |
| Choline bitartrate | 2 | 2.5 | 2.5 | 2.5 | 2.5 |
| Energy (kcal/g) | 3.61 | 3.77 | 4.572 | 4.572 | 4.572 |
| Energy (% kcal) | |||||
| Carbohydrate | 67.5 | 68.7 | 47 | 47 | 47 |
| Fat | 12.5 | 12.2 | 40 | 40 | 40 |
| Protein | 20 | 19.1 | 13 | 13 | 13 |
| Fatty acid profile (g/kg) | |||||
| Caprylic acid (C8:0) | 0 | 0 | 0.075 | 2.3 | 7.3 |
| Capric acid (C10:0) | 0 | 0 | 0.063 | 1.8 | 5.9 |
| Lauric acid (C12:0) | 0 | 0 | 0.45 | 13.5 | 43.3 |
| Myristic acid (C14:0) | 0 | 0.004 | .23 | 5.5 | 17.1 |
| Palmitic acid (C16:0) | 5.75 | 5.5 | 22.7 | 26 | 28.3 |
| Palmitoleic acid (C16:1) | 0 | 0.4 | 1.7 | 2 | 2 |
| Stearic acid (C18:0) | 0 | 1.05 | 4.6 | 8.5 | 12.7 |
| Oleic acid (C18:1) | 13.3 | 27.1 | 108.7 | 88 | 48.5 |
| Linoleic acid (C18:2) | 29.4 | 13.2 | 52.9 | 43.2 | 24.5 |
| α-Linolenic acid (C18:3) | 0.40 | 0.66 | 2.6 | 2.2 | 1.2 |
| Arachidic acid (C20:0) | 0.10 | 0 | 0 | 0 | 0 |
| % of total calories from SFA | 1.5% | 1.7% | 6% | 12% | 24% |
| % of total calories from MCFA (C6:0–C12:0) | — | — | 0.1% | 3.6% | 11.8% |
| % of total calories from LCSFA (C14:0–C20:0) | 1.5% | 1.7% | 5.9% | 8.4% | 12.2% |
| % of total calories from USFA | 11% | 10.5% | 34% | 28% | 16% |
| % of total calories from MUFA | 3.3% | 7% | 22.6% | 18.6% | 10.6% |
| % of total calories from PUFA | 7.6% | 3.5% | 11.4% | 9.4% | 5.4% |
| % of total calories from n-3 FA | 0.1% | 0.16% | 0.53% | 0.45% | 0.25% |
| % of total calories from n-6 FA | 7.5% | 3.2% | 10.8% | 8.9% | 5.1% |
| Cholesterol (mg/kg) | 0 | 0 | 3 | 34 | 65 |
| Ratio: MUFA:PUFA | 1:2.2 | 2:1 | 2:1 | 2:1 | 2:1 |
| Ratio: n-6:n-3 FA | 75:1 | 20:1 | 20:1 | 20:1 | 20:1 |