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. 2013 Jan;54(1):34–43. doi: 10.1194/jlr.M023846

Fig. 4.

Fig. 4.

mRNA and protein expression levels of ACAT1 in THP-1 macrophages and HMDMs exposed to 100 μg/ml ox-LDL in the absence (macrophages and macrophages exposed to 100 μg/ml ox-LDL or the 300 nM hKv1.3-E314 antibody alone) or presence of the hKv1.3-E314 antibody at varying concentrations of 37.5, 75, or 300 nM. In the presence of the 300 nM hKv1.3-E314 antibody, macrophages were exposed to 100 μg/ml ox-LDL for 24, 36, or 48 h. mRNA and protein levels of ACAT1 were assayed, respectively, by real-time quantitative RT-PCR and Western blotting. A and C: mRNA levels of ACAT1 in THP-1 macrophages and HMDMs,. B and D: Protein levels of ACAT1 in THP-1 macrophages and HMDM cells (n = 3). *P < 0.05, **P < 0.01, and P > 0.05 versus macrophages exposed to 100 μg/ml ox-LDL alone. There was no significant difference in mRNA and protein levels when macrophages were exposed to 100 μg/ml ox-LDL for 24, 36, or 48 h in the presence of the 300 nM hKv1.3-E314 antibody.