Fig. 2.

Overexpressed ADA-FoxO1 abrogates Elovl5 suppression of Pck1 and G6Pc in HepG2 cells. HepG2 cells were infected with Ad-Luc (control virus) and Ad-Elovl5 in the absence and presence of Ad-ADA-FoxO1 for 48 h. ADA-FoxO1 is insensitive to phosphorylation control (29). As in Fig. 1, cells were serum starved overnight and harvested the following day for RNA extraction and quantitation of Pck1 (A), G6Pc (B), and cyclophilin mRNA by qRT-PCR. Results are representative of three separate studies; results are expressed as mean ± SD, n = 3. * P ≤ 0.05 versus Ad-Luc; # P ≤ 0.05 versus no Ad-ADA-FoxO1 (None).