Fig. 5.

Diet and Elovl5 regulate mTOR, rictor, and raptor protein abundance in mouse liver. C57BL/6J mice were fed a low-fat diet (10% calories as fat; lean) or a high-fat diet (60% calories as fat; obese) for 12 weeks. Mice were infected with Ad-Luc or Ad-Elovl5 5 days prior to termination of the experiment. Five days after infection, mice were fasted overnight and euthanized the following day (Materials and Methods) (2). Hepatic protein extracts were assayed for protein levels of mTOR, mTOR-S²⁴⁴⁸ phosphorylation, rictor, raptor, mSin1, GβL, and Na,K-ATPase. A: Representative immunoblots (n = 3) separate mouse liver extracts per treatment. B: Phosphorylation status of mTOR-S²⁴⁴⁸; hepatic protein abundance of rictor (C) and raptor (D). Results are from two separate studies and are expressed as mean ± SD, n = 6. * P ≤ 0.05 versus lean Ad-Luc; # P ≤ 0.05 versus obese Ad-Luc.