Figure 7.

Antibody targeting of sAPP-α promotes amyloidogenic APP processing in vivo. PSAPP mice (3 female mice per group) at 8 months of age were intracerebroventricular (i.c.v.) injected with 2B3 antibody or isotype-matched IgG_2b control antibody at 5 μg/mL and sacrificed at 48 h after the injection. (a) Mouse brain homogenates were prepared from these mice and subjected to IB analysis for APP processing. IB using 6E10 antibody shows total APP and three bands corresponding to β-CTF and soluble Aβ species 38 and 40. Densitometry analysis shows the ratios of Aβ₄₀ to β-actin (b) and β-CTF to β-actin (c). A t test revealed significant differences in either ratio of Aβ₄₀ to β-actin or β-CTF to β-actin between 2B3 antibody- and control IgG_2b antibody-i.c.v. injected PSAPP mice. (***P < 0.001)