Table 4.
Biochemical properties of L-arabinose isomerases from various microorganisms
| Organism | Molecular mass (kDa) | Oligomeric state | Topt(°C) | pHopt | Metal ion requirement | References |
|---|---|---|---|---|---|---|
|
Arthrobacter sp. 22c |
335 (55.2)a |
6 |
47-52 |
5.0-9.0 |
Not required |
This study |
|
Lactobacillus sakei 23 K |
220 (53.7)a |
4 |
30-40 |
5.0-7.0 |
Mn2+, Mg2+ |
32 |
|
Escherichia coli |
362 (56.0)a |
6 |
30 |
8.0 |
Fe2+, Mn2+ |
18, 37 |
|
Bacillus halodurans |
224 (56.3)a |
4 |
50 |
7.5-8.0 |
Not required |
19 |
|
Lactobacillus plantarum NC8 |
342 (53.6)a |
6 |
60 |
7.5 |
Mn2+, Co2+ |
20 |
|
Thermus sp. IM6501 |
(56.0)a |
NR |
60 |
8.5 |
Mn2+ |
27 |
|
Lactobacillus fermentum CGMCC2921 |
(53.4)a |
NR |
65 |
6.5 |
Mn2+, Co2+ |
21 |
|
Bacillus stearothermophilus IAM 11001 |
(55.9)a |
NR |
65 |
7.5 |
Mn2+ |
24 |
|
Alicyclobacillus acidocaldarius |
224 (56.0)a |
4 |
65 |
6.0-6.5 |
Mn2+, Co2+, Mg2+ |
25 |
|
Thermoanaerobacter mathranii |
220 (52.8)a |
4 |
65 |
8.0 |
Mn2+ |
29 |
|
Geobacillus stearothermophilus |
224 (56.1)a |
4 |
70 |
7.0-7.5 |
Mn2+, Co2+, Mg2+ |
19 |
|
Geobacillus thermodenitrificans |
230 (56.1)a |
4 |
70 |
8.5 |
Mn2+ |
22 |
|
Acidothermus cellulolytics ATCC 43068 |
(54.8)a |
NR |
75 |
7.5 |
Mn2+, Co2+ |
28 |
|
Bacillus stearothermophilus US100 |
225 (56.2)a |
4 |
80 |
7.5-8.0 |
Mn2+b, Co2+b |
23 |
|
Thermotoga neapolitana |
(56.7)a |
NR |
85 |
7.0 |
Mn2+, Co2+ |
30 |
|
Thermotoga maritima |
230 (56.7)a |
4 |
90 |
7.5 |
Mn2+, Co2+ |
31 |
| Anoxybacillus flavithermus TC-06 | (55.9)a | NR | 95 | 9.5-10.5 | Ni2+ | 26 |
NR not reported; a molecular mass of the monomer, calculated from the amino acid sequence; b metal ions required only for thermostability [37].