Table 1.

Summary of transgenic and mutant Arabidopsis lines characterized in this study

Plant Lines	Construct, key element and genetic background	Gene expression vs. WT control	Key phenotypes
sk156	pSKI015, 35S enhancer, Col-4 background	91.4- and 95.8-fold higher expression for miR156b, At4g30975	Increased seed carotenoids and branching
S468	pROK2, SALK_032468, Col-0 background	11.1 fold less expression for AT4G30980	Similar to WT
35S:bHLH069	Modified pBI121, 35S:At4g30980 cDNA, sk156 background	708.4 fold higher expression for bHLH069	Similar to sk156
T156b	Modified pBI121, 35S:miR156b, Col-4 background	2134.6 fold higher expression for miR156b	Similar to sk156
A975	Modified pBI121, 35S:At4g30975 cDNA, Col-4 background	332.5 fold higher expression for At4g30975	Similar to WT
CS2117	pDs-Lox, CS852117, Col background	4.3 fold less expression for SPL15	Slightly stunted cauline stem and increased seed carotenoids.
Col background	4.3 fold less expression for SPL15	Slightly stunted cauline stem and increased seed carotenoids.
CS6815	pDs-Lox, CS856815, Col background	5.2 fold less expression for SPL15	Similar to CS2117.
S8712	pROK2, SALK_138712, Columbia background	4.7 fold less expression for SPL15	Similar to CS2117.
35S:SPL15n	Modified pBI121,35S:SPL15n (miR156 sensitive), sk156 background	4.7 fold less expresson for SPL15.	Similar to sk156
35S: SPL15m	Modified pBI121, 35S:SPL15m (miR156 insensitive), sk156 background	22.8-613.6 fold higher expression of SPL15	Similar to WT, changed leaf shape.
AS1:SPL15n	Modified pBI121, AS1:SPL15n (miR156 sensitive), sk156 background	3.2 fold less expression for SPL15	Similar to sk156, slightly changed leaf color
AS1:SPL15m	Modified pBI121,AS1:SPL15m (miR156 insensitive), sk156 background	4.6 fold higher expression for SPL15	Similar to WT, changed leaf shape
SPL15:SPL15m	Modified pBI121, SPL15:SPL15m (miR156 insensitive), sk156 background	1.6 fold higher expression for SPL15	Similar to sk156, changed leaf shape