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Transcriptional regulation of autophagic genes by fenugreek. Jurkat cells incubated with 250, 500 and 1000 μg/mL of fenugreek extract were quantitatively analyzed for transcripts of autophagic pathway genes beclin 1, ATG5 and LC3 by RT-PCR using β-actin as internal reference. Dose dependent increase in LC3 transcripts was found in cells incubated for 48 hr while no significant changes were observed for beclin 1 and ATG5.
