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Far-Western analysis of PlcR-Fnr, PlcR-ResD and ResD-Fnr interactions. Increased amounts of purified Fnr, ResD and PlcR were spotted onto nitrocellulose membranes and incubated with biotinylated-PlcR (A) or biotinylated-ResD (B), under anoxic conditions. PlcR and ResD binding was detected using streptavidin-HRP complex and visualized by chemiluminescence. BSA was used as negative control.
