Figure 8.

Solubility and conformational properties of Sup35-NM as a function of the temperature. (A) Western blot of the soluble and insoluble fractions of cells expressing Sup35-NM at 18 and 37°C detected with anti-histag antibody and quantified by Quantity One software. (B) Comparative analysis of the secondary structure of Sup35-NM IBs formed at 18°C and 37°C as determined FT-IR spectroscopy in the amide I region of the spectrum. Empty circles, solid thick lines and solid thin lines show the absorbance spectra, the sum of individual spectral components and the deconvolved component bands, respectively.