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. 2012 Nov 9;7:36. doi: 10.1186/1749-8104-7-36

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Copyright ©2012 Philipp et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Robo1 localizes to Rab11-positive vesicles. Myc-tagged Robo1 was expressed in HEK293 cells. Antibodies recognizing marker proteins for early endosomes (EAA1) and lysosomes (LAMP1), as well as Rab7 (late endosomes) and Rab11 (labeling recycling endosomes) were used to analyze Robo1 localization in the cell. Robo1 (A) was found to co-localize considerably with EAA1 (B and C). No overlap was seen between Robo1 (D) and LAMP1 (EF). Similarly, Robo1 (G) did not co-localize with Rab7 (HI), indicating that Robo1 was not shuttled to the late endosome/lysosome compartment. Almost complete overlap was found between Robo1 (J) and Rab11 (KL), suggesting that Robo1 was predominantly stored in Rab11-positive vesicles which have been shown to be involved in exocytosis and regulated secretion [58,69] .