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. 2012 Dec 12;7(12):e52053. doi: 10.1371/journal.pone.0052053

Table 1. Primer sequences used for PCR or real-time PCR.

Target genes Forward primer (5′ →3′) Reverse primer (5′ →3′) Products (bp)
CYP27A1 GCTCTTGGAGCAAGTGATG AGCATCCGTATAGAGCGC 196
CYP2R1 TTGGAGGCATATCAACTGTGGT CTCGGCCATATCTGGAATTGAG 153
GAPDH GAAGGTGAAGGTCGGAGTC GAAGATGGTGATGGGATTTC 226