Table 1. Primer sequences used for PCR or real-time PCR.
Target genes | Forward primer (5′ →3′) | Reverse primer (5′ →3′) | Products (bp) |
CYP27A1 | GCTCTTGGAGCAAGTGATG | AGCATCCGTATAGAGCGC | 196 |
CYP2R1 | TTGGAGGCATATCAACTGTGGT | CTCGGCCATATCTGGAATTGAG | 153 |
GAPDH | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC | 226 |