Figure 7. Isoflurane effects on the expression of NeuN, drebrin and synaptophysin in rat brain tissues.

Eighteen-month-old Fisher 344 rats had the training sessions of fear conditioning and 30 min later were exposed to or were not exposed to 1.2% isoflurane in the presence or absence of lidocaine for 2 h. Hippocampus and cerebral cortex were harvested at 48 h after anesthetic exposure for Western blotting. A: representative Western blot images. B, C and D: graphic presentation of the NeuN, drebrin and synaptophysin protein abundance quantified by integrating the volume of autoradiograms from 4 rats for each experimental condition. Values in graphs are expressed as fold changes over the mean values of control animals and are presented as the means±S.D. C: control, I: isoflurane, I+L: isoflurane plus lidocaine.