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. 2012 Dec 12;7(12):e51423. doi: 10.1371/journal.pone.0051423

Figure 8. Chloroquine abolished effects for TLR9 agonists on IgM secretion in HeLa MR cells.

Figure 8

A, semi-quantative RT-PCR showed increased Ig µ expression after stimulation by CpG 2006, CpG 2078 and GpC (upper panel). The effects of TLR9 agonists were abolished by chloroquine (lower panel). GAPDH, internal control. B, flow cytometry analysis showed that the cytoplasmic IgM level was decreased after stimulation with CpG 2006, CpG 2078 and GpC, and that this was abolished by chloroquine. *P<0.05 vs. vehicle control; **P<0.01 vs. vehicle control. C, Western blot analysis showed that the cytoplasmic IgM level was decreased after stimulation with CpG 2006, CpG 2078 and GpC, and that this was abolished by chloroquine. D, Western blot analysis showed that IκB was phosphorylated after stimulation with CpG 2006, CpG 2078 and GpC, and that this was abolished by chloroquine.