Figure 1. Increase of CD4⁺CD25^highCD127⁻ Treg cell numbers following G-CSF mobilization.

Cell numbers of PBMCs isolated by Ficoll density gradient (A) MACS isolated CD4⁺ T cells (B) and subsequently FACS isolated CD4⁺CD25^highCD127- Tregs (C) per µl blood withdrawal volume before (n = 11, pre) and after (n = 22, post) G-CSF mobilization. Cell numbers of PBMCs isolated by Ficoll density gradient (D) MACS isolated CD4+ T cells (E) and subsequently FACS isolated CD4⁺CD25^highCD127⁻ Tregs (F) per µl of the total leukapheresis product. Black lines indicate median values.