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. 2012 Dec 12;7(12):e51644. doi: 10.1371/journal.pone.0051644

Figure 3. Th1 associated migration capacity of donor Tregs before and after SC mobilization.

Figure 3

PBMCs isolated from stem cell donors before (n = 6) and after (n = 13) SC mobilization were stained with anti human CD4 and CD25 antibodies and pretreated with 300 ng IP10, the ligand of CXCR3. Subsequently cells were stained with anti human Foxp3 A647 and Phalloidin FITC in order to measure induction of F-actin polymerization as a consequence of stimulation with the corresponding ligand. Data are shown as fold change of Phalloidin detection and presented as box whisker plots.