Figure 2. Identification of the transcription starting site by 5′RACE PCR.

(A) Agarose gel showed multiple 5′RACE PCR products. (B) Mapping the potential TSS of the DRAM gene. The arrow indicated the nested reverse 5′RACE primer. The GeneRacer PCR, based on RNA ligase-mediated and oligo-capping rapid amplification of cDNA, was carried out according to the manufacturer's instructions. The PCR products were resolved on agarose gel, and potential bands were collected and confirmed by DNA sequencing. * indicates a non-specific amplification of the primer dimmers.