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. 2012 Dec 12;7(12):e50502. doi: 10.1371/journal.pone.0050502

Figure 3. The proximal region of the DRAM promoter is sensitive to nuclease digestion.

Figure 3

(A) Schematic representation of the primer sets used for CHART-PCR. (B) R6 region of the DRAM promoter is more sensitive to DNase I and MNase digestion. The genomic DNA was isolated from Hep3B and HepG2 cells and analyzed by quantitative PCR. The data was shown as percentage of digested DNAs to undigested DNAs. (C) Serum deprivation increases the accessibility of R5, R6 and R7 regions to nuclease digestion in Hep3B and HepG2 cells.