Figure 4. Serum deprivation induces changes in histone modification at the DRAM promoter locus in Hep3B cells.

Hep3B cells were prepared for chromatin IP (ChIP) assays. The chromatin DNAs were immunoprecipitated with antibodies specific to H3K4me3 (A), anti-di-acetyl-H3 (B), anti-H3K9me2 (C) and anti-tetra-acetyl-H4 (D), and the enriched DNA fragments flanking the DRAM promoter were analyzed by quantitative PCR. Data were presented as the amount of DNA recovered by specific antibodies relative to DNA enriched by the appropriate IgG controls. The results were expressed as the means±standard deviations of three independent experiments. *: P<0.05.