Figure 5. The putative cis regulatory elements responsive to serum deprivation is resided in the DRAM promoter region flanking −19/+28 nucleotides.

(A) Hep3B and HepG2 cells were transiently transfected with reporter plasmids containing truncated versions of the promoter region of the DRAM gene as indicated. Luc-DCP is defined as the reporter containing the shortest promoter region (−19∼+28). (B) The core promoter region contains a putative NF-κB binding site. Transcription factor binding sites presented in the core promoter region (-19∼+28) were predicated by web software TFSEARCH and MatInspector and the mutation or deletion plasmids were generated using the site-directed mutagenesis method. (C) Mutation or deletion of the NF-κB binding site does not affect the core promoter activity.