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. 2012 Dec 12;7(12):e50850. doi: 10.1371/journal.pone.0050850

Figure 3. Effect of CB1-RNAi-LV on the proliferation of primary hepatic stellate cells.

Figure 3

(A) The statistical results of the optical density (OD) value of BrdU incorporative cells in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by ELISA method. “# #” p<0.01. Results showed that the proliferation of HSCs stimulated by PDGF-BB elevated significantly than HSCs alone. NC-LV transfection did not affect the proliferation of HSCs stimulated by PDGF-BB. Furthermore, the proliferation of HSCs stimulated with PDGF-BB and transfected with CB1-RNAi-LV decreased markedly than that of HSCs stimulated with PDGF-BB and transfected with NC-LV. (B) The statistical results of PDGFR-β mRNA expression in HSCs (named Con), HSCs with PDGF (named Con+PDGF), HSCs with NC-LV and PDGF (named NC+PDGF), HSCs with CB1-RNAi-LV and PDGF (named siRNA+PDGF) by RT-PCR analysis. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. “# #” p<0.01. Results showed that PDGFR-β mRNA expression increased markedly by PDGF-BB pretreatment or by PDGF-BB pretreatment plus NC-LV transfection, while PDGFR-β mRNA expression in HSCs pretreated with PDGF-BB and transfected with CB1-RNAi-LV decreased markedly than that in HSCs pretreated with PDGF-BB and transfected with NC-LV.