Skip to main content
. 2012 Dec 12;7(12):e50817. doi: 10.1371/journal.pone.0050817

Figure 4. PCR analysis of genomic DNA extracted from semen of transgenic germ line chimeric (G0) quail.

Figure 4

(A) PCR product of eGFP; (B) PCR product of housekeeping gene GAPDH; (1–5) PCR products of sexually matured G0 males produced by microinjection to blood vessels of embryo at HH Stage 13–15 (6–8). PCR analysis of three sexually mature males produced by microinjection beneath the subgerminal cavity of the blastodermal embryo. (M) Standard DNA markers. (P) Positive control. (N) negative control without microinjection of lentiviral vector. PCR product size is indicated on the right.