Skip to main content
. 2012 Dec 12;7(12):e48637. doi: 10.1371/journal.pone.0048637

Figure 1. Multiple members of SEMA3/Plexin ligand-receptor complexes expressed in human PDAC tissues and cell lines.

Figure 1

(A) immunohistochemistry of human PDAC cases showing expression of NRP1 and most members of SEMA3/Plexin family. Normal pancreatic tissue did not express any SEMA3/Plexin/NRP (diagonal, scale bar = 50 µm). Scale bar = 100 µm. (B) Bar graph to show distribution of numbers of SEMA3/Plexin/NRP expressed in each PDAC case. Fifty-three out of 62 cases (85.5%) expressed more than 5 members. (C) semi-quantitative RT-PCR analysis of SEMA3-, Plexin- and NRP- transcripts in human PDAC cell lines. GAPDH served as an internal loading control. (D) immunoblot of the whole-cell lysate from human PDAC cell lines to detect endogenous SEMA3, Plexin, and NRP1. (E) representative SEMA3-, Plexin- and NRP1-immunoreactivity detected in PanIN lesions adjacent to invasive carcinoma. Scale bar = 100 µm.