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. 2012 Dec 12;7(12):e51215. doi: 10.1371/journal.pone.0051215

Figure 5. iNKT cells do not directly suppress antigen-specific CD4+ T cells in vitro.

Figure 5

Mice were immunized with GPI peptide and treated with either DMSO or α-GalCer. Mice were euthanized on day 10 and dLNs were harvested. (A) Total CD4+T cells (CD3+ CD4+ cells) and NKT-depleted CD4+T cells (CD3+ CD4+ α-GalCer loaded CD1d-tetramer negative cells) were sorted by flow cytometry. These cells were cultured with sorted MMC-treated CD3-negative cells as APC in the presence of 10 µM of GPI peptide for 24 h. (B) Supernatants were collected and subjects to quantitative analysis of IL-17 and IFN-γ levels by ELISA. Data are mean±SD. (n = 4–5). *p<0.05, **p<0.01, by Man-Whitney analysis.