Figure 2. Myo7a mutations identified in Myo7a^I487N/I487N ewaso and Myo7a^F947I/F947I dumbo strains.

(A) Direct sequencing identified a 1460T>A (I487N) missense mutation in Myo7a^I487N/I487N ewaso and a missense mutation 2839T>A (F947I) in the Myo7a^F947I/F947I dumbo mouse strain (B). Wildtype and heterozygote sequences are shown for comparison. (C) Sequence conservations of Myo7a^I487N/I487N ewaso (red) and Myo7a^F947I/F947I dumbo (blue) mutations, and the closely positioned Myo7a^sh-1 shaker mutation (green) showing high evolutionary conservation of our two mutant strains.