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. 2012 Dec 12;7(12):e51270. doi: 10.1371/journal.pone.0051270

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© 2012 Jensen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Tg(Xla.rho:rtTA, TRE:GFP); alb^−/− embryos were reared to 6 dpf. Larvae were treated with 10 µg/mL doxycycline (Dox) for the durations indicated prior to fixation at 6 dpf. Fixed larval tissues were processed for cryosectioning and immunofluorescence with antibodies against GFP and Rhodopsin. GFP-positive and Rhodopsin-positive rod outer segments were counted and averaged amongst 3 confocal z-projections for each of the 2–72 h treatment groups. The ratio of GFP-positive to Rhodopsin-positive cells is plotted for each treatment group; error bars represent standard deviation. () The untreated and 1 h Dox-treated samples were quantified by counting the number of GFP-positive cells in all retinal sections from 10 eyes and dividing by an estimation of the total number of rods per eye. Statistical comparisons are indicated by brackets. ***, p<0.001; **, p<0.01; NS, not significant.

Inline graphic — Tg(Xla.rho:rtTA, TRE:GFP); alb^−/− embryos were reared to 6 dpf. Larvae were treated with 10 µg/mL doxycycline (Dox) for the durations indicated prior to fixation at 6 dpf. Fixed larval tissues were processed for cryosectioning and immunofluorescence with antibodies against GFP and Rhodopsin. GFP-positive and Rhodopsin-positive rod outer segments were counted and averaged amongst 3 confocal z-projections for each of the 2–72 h treatment groups. The ratio of GFP-positive to Rhodopsin-positive cells is plotted for each treatment group; error bars represent standard deviation. () The untreated and 1 h Dox-treated samples were quantified by counting the number of GFP-positive cells in all retinal sections from 10 eyes and dividing by an estimation of the total number of rods per eye. Statistical comparisons are indicated by brackets. ***, p<0.001; **, p<0.01; NS, not significant.