Figure 6. Combination of HSVtk expression and GCV induces Grp78 promoter.
9L cells stably transduced with (A) RGD4C/Grp78-HSVtk or (B) RGD4C/CMV-HSVtk were treated with GCV for the indicated times, then subjected to western blot analyses with antibodies against Grp78, HSVtk and GAPDH as control. Endogenous Grp78 and HSVtk levels were measured, normalized to GAPDH and fold increase over time zero-control was calculated for each sample (n=3). C) and D) Western blot analysis of phospho-eIF2α and ATF6 expression, respectively.