Table 2.
Biochemical properties and immunoreactivity pattern of the proteins. Dot-blot assay was performed for each second dimension fraction containing the proteins identified. The reactivity was quantified into pixels unit and graded according to reactivity intensity with respect to that of the crude parasite antigen to obtain relative reactive intensity. Using the relative reactive intensity, each reactive spot was scored as ‘weak reactivity’ (−), ‘moderate reactivity’ (±) or ‘strong reactivity’ (+). In addition to reactive pattern, proteins were characterized using GRAVY index (ProtParam tool), number of antigenic determinant contained in the full amino acid length and the antigenic propensity where >1 indicates high antigenic character. In this Table antibody reactivity pattern is not repeated for proteins from the same fraction well.
| Fraction Well | Protein identified | GRAVY index | Amino acids | Antigenic determinants | Antigenic propensity | Antibody reactivity pattern | |||
|---|---|---|---|---|---|---|---|---|---|
| IgE | IgG1 | IgG3 | IgG4 | ||||||
| F2-C3 | Chromatin licensing/DNA replication factor 1 | −0.348 | 578 | 20 | 1.0414 | + | − | − | + |
| F2-C5 | Zincfinger, RanBP2-type,domain-containing | −0.986 | 513 | 21 | 1.0108 | + | − | − | ± |
| F2-C7 | Zinc finger, RanBP2-type,domain-containing | −0.986 | 513 | 21 | 1.0108 | ± | − | − | ± |
| F3-E1 | Meiosis-specific, nuclear structural protein 1 | −1.306 | 376 | 11 | 0.9936 | + | ± | + | + |
| Chromatin licensing/DNA replication factor 1 | −0.348 | 578 | 20 | 1.0414 | + | ± | + | + | |
| NACHT/WD repeat domain-containing protein 1 | −0.282 | 2005 | 81 | 1.0271 | |||||
| Triple functional domain protein | −0.414 | 1554 | 68 | 1.0336 | |||||
| RNA Helicase [EC:3.6.1.-] | −0.409 | 762 | 25 | 1.0308 | |||||
| Protein kinase [EC:2.7.1.-] | −0.22 | 1921 | 63 | 1.0397 | |||||
| BRO1 domain-containing protein BROX | −0.177 | 426 | 16 | 1.0459 | |||||
| Cell division control protein CDC7 | −0.333 | 330 | 13 | 1.0363 | |||||
| Centriolin | −0.847 | 2444 | 92 | 1.0139 | |||||
| Small conductance calcium-activated potassium channel protein 2, putative | −0.393 | 1536 | 58 | 1.0294 | |||||
| CREB-binding protein | −0.708 | 986 | 29 | 1.0269 | |||||
| PDZ and LIM domain protein 3 | −0.674 | 317 | 13 | 1.0199 | |||||
| Prolyl-tRNA synthetase [EC6.1.1.15] | −0.473 | 1135 | 41 | 1.0295 | |||||
| F3-H7 | Zinc finger, RanBP2-type, domain-containing | −0.986 | 513 | 21 | 1.0108 | − | − | ± | − |
| F18-B1 | E3 ubiquitin-protein ligase HUWE1 | −0.423 | 2720 | 105 | 1.0231 | − | + | + | + |
| 5'-nucleotidase, cytosolic II | −0.2 | 476 | 21 | 1.0368 | |||||
| F18-C3 | Chromatin licensing/DNA replication factor 1 | −0.348 | 578 | 20 | 1.0414 | − | + | + | + |
| Ubiquitin-conjugating enzyme E2 N | −0.228 | 173 | 8 | 1.0386 | |||||
| F31-C3 | Chromatin licensing/DNA replication factor 1 | −0.348 | 578 | 20 | 1.0414 | − | + | + | + |
| F31-E1 | Putative serine/threonine-protein kinase C05D10.2 | −0.774 | 1061 | 39 | 1.0118 | + | + | + | + |
| F31-H7 | Chromatin licensing/DNA replication factor 1 | −0.348 | 578 | 20 | 1.0414 | − | − | − | − |
| NACHT/WD repeat domain-containing protein 1 | −0.282 | 2005 | 81 | 1.0271 | |||||